Scientists of National Institute of Cholera and Enteric Diseases (NICED) are working on different aspects of diarrheal diseases with special emphasis on development of vaccines against enteric diseases. Our Institute also raises and supplies antisera for detection and serotyping of Vibrio cholerae strains. NICED Animal Facilities division is engaged in care, breeding and management of animals required for studies conducted by researchers of the Institute.
The Micro Ventilated Cage System: Modern animal house facility at NICED was developed in collaboration with JICA project. This facility houses special animal cage system in which the air flow is maintained through the enclosures by a pressure differential system between the cage chamber and the exhaust chamber. This is accomplished by maintaining the cage chamber at a positive pressure with respect to the enclosures and maintaining the exhaust chamber at a negative pressure with respect to the enclosures. Both the input and the exhausted air are sterilized by various devices in the path of the ventilation stream. Purpose is to keep the animal germ free. The optimum humidity and temperature of the cage chamber is maintained.
Surgical room is equipped with modern instruments like radio frequency operated surgical knife, anesthesia maintaining ventilation device, Vacuumed operated animal hair clipping machine etc which are used during operation and immunization to give minimum pain to the animal.
Successful management of our animal resource facilities involves effective utilization of manpower to achieve the goal of comprehensive animal care. We have trained and experienced staff to look after the animals. This facility has been given the responsibilities of production and maintenance of laboratory animals for research.
Recently we are having the following laboratory animal species and strains:
|Rabbit||New Zealand White||Outbred|
|Guinea Pig||English Albino||Outbred|
In wake of increasing threat of highly pathogenic viral infections such as Avian Influenza, Ministry of Health and Family Welfare provided funds in 2006 for setting up a BSL3 laboratory at NICED to cater to the Eastern states of India. A modular prefabricated BSL3 laboratory was installed and inaugurated at NICED in 2007 in the ID and BG hospital Campus by Director General, ICMR in the presence of Additional Chief Secretary, Govt of West Bengal and other dignitaries from the State Health Department. BSL-3 is suitable for work with infectious agents which may cause serious or potentially lethal diseases as a result of exposure even through aerosol. During the Avian Influenza outbreak in poultry in West Bengal during January-February 2008 and Influenza A/H1N1/ 2009 pandemic during July-Dec 2009 all suspected human samples from West Bengal and neighboring states were handled and analyzed by Real time PCR in the BSL3 laboratory. Subsequently the lab has also been utilized during INFA/H1n!/ 2009 outbreak during 2015 for providing laboratory services to the state. This facility is under the Division of Virology.
The Institute has a central instrumentation facility located in the 3rd floor of S. C. Pal Building. Due to recent expansion, another set up will be coming up soon in the 2nd floor of JICA building. The central facility is equipped with high-speed centrifuges, ultracentrifuges, spectrophotometer, sonicator, and shakers for large bacterial cultures. This facility is being upgraded time to time. Recently, a large number of sophisticated instruments have been procured and these are placed under scientists for proper maintenance and utilization. These include FPLC, HPLC, LC-MS, calorimeters like ITC and DSC, FACS, confocal microscope, electron microscope, real-time PCR and automated DNA sequencer. The instruments are available for use mainly by our scientists and our collaborators.
Flow Cytometry Core Facility:
NICED established its Flow cytometry(Fluorescence activated Cell Sorting or FACS) as central Instrument facility in 2010. Flow cytometry is a laser-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspendingcells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. It is routinely used in the diagnosis of health disorders, especially blood cancers/ HIV etc. NICED facility has FACSAria II flow cytometer with 9 colour (2 near ultra violet, 5 blue and 2 red) three laser system with 375nm near UV, 488nm blue and 633nm red laser. The lasers are contained and the laser energy is rendered within the instrument structure making it completely safe during its functioning. No special work area safety is required. Structurally, it has three main components - (i) fluidics cart (pumps provide 5 - 75 psi pressure to accommodate a variety of cell sorting), (ii) optics system (comprising of lasers, fiber optics, optic filters and photomultiplier tubes (PMTs)) and (iii) cytometer electronics (converting optical signal to digital signal). This set up is highly compatible for both the purposes of analysis and sorting
The division has the following equipments:
The Biomedical Informatics Centre (BIC) of National Institute of Cholera and Enteric Diseases, Kolkata was established in July, 2006. This is the one of the 20 centres created by the Indian Council of Medical Research (ICMR), Govt. of India with a mission to ensure balanced and integrated development of information dissemination, application development and R&D in biomedical informatics.