National Institute of Cholera and Enteric Diseases

Indian Council of Medical Research
Department of Health Research, Ministry of Health and Family Welfare, Government of India
WHO Collaborating Centre for Research and Training on Diarrhoeal Diseases

NICED : Scientists

Dr. Hemanta Koley

Dr. Hemanta Koley

Field of Research:

Research focus is on the development of novel vaccine candidate against enteric pathogens like Vibrio cholerae, Shigella, Salmonella, Escherichia coli, and Campylobacter for the livestock and human.
Laboratory has identified a number of potential vaccine candidates like outer membrane vesicles, heat killed bacteria, live attenuated bacteria, chitosan-alginate nanoparticle.
Special expertise on outer membrane vesicles based studies on various animal models, like Mouse, Rat, Guinea pig, Rabbit, Chicken and Zebra fish.
Long path programme of the laboratory is to develop a single vaccine candidate against all enteric pathogens.
 

Educational Background:

In 1992, after completion of Master degree in Human Physiology from Dept. of Physiology, University College of Science & Technology, Calcutta University, he joined Division of Bacteriology, National Institute of Cholera and Enteric Diseases (NICED), an integral Institute of the Indian Council of Medical Research and a WHO Collaborating Centre for Research and Training in Diarrhoeal Diseases, Kolkata, India, for Ph. D. degree under supervision of Dr. GB Nair. In 1996, he joined as Associate Professor in Dept. of Physiology, College of Medical Sciences-Nepal, where he devoted himself entirely in teaching medical students. Meanwhile, Dr. Koley got an opportunity for Post-Doctoral research in the laboratory of Prof Dipak K Sarkar, Dept Animal Sciences, Rutgers - The State University of New Jersey, New Brunswick, USA. After completion of one year in Rutgers University, Dr. Koley moved to Boston, joined Gastroenterology Section of Harvard Medical School, as Research Associate and worked there on "Molecular mechanism of Barrett's oesophagus and to identify therapeutic target for Adenocarcinoma" under leadership of Prof. R K Goyal, renowned gastroenterologist.

Research Achievement:

Published 70 original papers and 40 abstracts in his credit.

Secured patent for a process for the preparation of Cholera Vaccine-VA1.3 (European Patent Office; Patent No.- 97309957.5-2105 on 19/05/98) in pre-doctoral research in NICED

Received Young Scientist Award in IUPS International Congress, Christchurch, New Zealand, August 2001.

Awarded Oration Lecture: "Dr. (Mrs.) Chitralekha Mukherjee Memorial Oration-2010" and given lecture at St. John's Medical College, Bangalore on 16th December 2010

Recently developed Shigella Vaccine and applied patent through Indian council of Medical Research, New Delhi. Patent application for: "A multiple outer membrane vesicles (MOMV) of Shigellae as a Novel Candidate vaccine" ICMR New Delhi. (Patent application no. 1652/Del/2013 dated 31.05.2013)

International patent application: PCT patent application titled A Multi-Serotype Outer Membrane Vesicles (MOMV) of shigellae as a novel candidate vaccine' (PCT/IN2014/000369).

Patent application for: "Alginate chitosan Nano formulation of Omp-A, a Shigella protein subunit vaccine" ICMR New Delhi. (Patent application # 201611015219/ IPR/NICED/OmpA/329 June, 2015)
Invited special lecture at "Asian Society of Diarrhoeal Disease and Nutrition" conference (ASCODD) at Kochi between October 30,2017 to November 1, 2017.

Invited Lecture on "Shigella Vaccine" Occasion of MSD-Hilleman Laboratory Science day workshop on 2nd Nov 2017 at Imperial Hotel, New Delhi.
 

General Information
Name Dr. Hemanta Koley
Educational Qualification M.Sc., Ph.D.
Designation Scientist E
Division Bacteriology
Specialization Bacteriologist
Date of joining ICMR 1st October 2004
Email : hemantakoley@hotmail.com

Profile

Professional Experience

1992-96 Joined as Junior Research Fellow worked on isolation, characterizations of clinical and environmental enteric organism and completed of Ph. D. degree from University of Calcutta.
1996-2001, joined as Associate Professor in Dept. Physiology, College of Medical Sciences-Nepal, where he devoted entire heartily in teaching medical
2001-2002 worked on Foetal Alcohol different modelsaspost-doctoral research associate at Dept of Animal Sciences, Rutgers -The State University of New Jersey, New Brunswick, USA.
2002-2004 joined Gastroenterology Section, Harvard Medical School as Research Associate and worked there on "Molecular mechanism of Barrett's oesophagus and to identify therapeutic target for Adenocarcinoma"
2004 - Still date: Joined back to Division of Bacteriology,National Institute of Cholera and Enteric Diseases (NICED), Kolkata, devotedly working on development of a single vaccine protect against entire circulating enteric organisms.
 

Research Interests

Research interest is to understand signal transduction pathways in immune and inflammatory cells during diarrhoea and also to study the nature of protection against diarrhoeal pathogens like Vibrio cholerae, Shigella, Diarrhoeagenic E Coli, Typhoidal and non Typhoidalsalmonellasp, Campylobactersp in different animal models.

Project Funding

Intramural Project

  1. Studies on colonization ability of tcp-ve Vibrio cholerae strains in animal models. ICMR-
  2. Studies on a novel approach of using outer membrane vesicles (OMVs) of Shigella as a candidate vaccine in animal models.
  3. Evaluation of immunogenicity and protective efficacy of ∆tolA Shigella OMVs in animal model
  4. Formulation of a novel Outer Membrane Vesicle (OMVs) based immunogen from S. Typhi and Paratyphi A and the induction of adaptive immunity and protective efficacy in mice model.

Extramural Project

  1. Development of Nanoparticle or MicroparticleAdjuvanted Subunit Oral Vaccine against Poultry Salmonellosis, DBT, New Delhi.
  2. Studies on immunogenicity and protective efficacy of multi-serotype OMVs of circulating Salmonella strains in chicken model, DST, Kolkata
  3. Development and evaluation of a heat killed multi-serotype oral Shigella vaccine- Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), Ministry of Education, Culture, Sports, Science and Technology of Japan.
  4. Development of shigella vaccine based on the virulence gene expression. Funded by Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan.
  5. Vaccination accination and protection studies for a targeted Nanoparticulate oral vaccine against shigelosis. DBT, New Delhi.

Alumni Ph.D. Students

  • Dr Nivedita Roy.
    Present affiliation: Post-Doctoral fellow. Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
  • Dr Soumik Barman.
    Past affiliation: Post-Doctoral fellow. Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
    Present affiliation: Post-Doctoral fellow. Department of Pediatrics, University of Washington School of Medicine, Seattle Children's Research Institute, Seattle, USA.
  • Dr Soma Mitra.
    Past affiliation: Post-Doctoral fellow. Department of Bacteriology, National Institute of Infectious Diseases (NIID), Japan.
    Present affiliation: ICMR Post-Doctoral fellow. Division of Bacteriology, ICMR-NICED.
  • Dr Dhrubajyoti Nag.
    Past affiliation: Post-Doctoral fellow. University of Kansas Medical Center. USA.
    Present affiliation: Post-Doctoral fellow. Wayne State University, USA.
  • Dr Ritam Sinha.
    Past affiliation: Research Associate, Department of Biological Sciences, IISER,India .
    Present affiliation: Post-Doctoral Research Associate, Department of Microbiology and Molecular Genetics, Michigan State University. USA.

Post-Doctoral fellows:

  • Dr Soma Mitra, ICMR Post-Doctoral fellow
  • Dr Samadrita Sengupta, ICMR Post-Doctoral fellow

PhD students:

  • Ms Priyadarshini Mukherjee, UGC Senior Fellow
  • Mr Debaki Ranjan Howlader,CSIR Senior Fellow
  • Ms Ushasi Bhaumik,DST, Junior Fellow
  • Mr Suhrid Maiti,CSIR, Project Fellow
  • Mr Vivek Mondal,CSIR Project Fellow
  • Mr Sounak Sarkar, Project Fellow

Technical Staff:

  • Mr SuhasitRanjan Ghosh, Technical Offer A

Collaborators:

National Level Collaboration:

  • Dr. Shantanu Tamuly
    Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-22. Assam
  • Dr. Devasish Chowdhury
    Physical Sciences Division
    Institute of Advanced Study in Science and Technology, Guwahati
  • Dr Dhirendra S. Katti
    Professor
    Department of Biological Sciences and Bioengineering
    Indian Institute of Technology - Kanpur
    Kanpur - 208016, UP
    INDIA

International Level Collaboration:

  • Dr Jiro Mitobe, M.D. - Ph.D
    Department of Bacteriology I
    National Institute of Infectious Diseases
    1-23-1 Toyama Shinjuku Tokyo 162-8640
    Japan
  • Dr Jeffrey H. Withey, Ph.D.
    Associate Professor
    Department of Immunology and Microbiology
    Wayne State University School of Medicine
    Detroit, MI 48201
    USA
  • Dr. Deanna L. Gibson, Ph.D.
    Associate Professor, Biology
    University of British Columbia, Okanagan campus
    250-807-8790 / deanna.gibson@ubc.ca
    3187 University Way, Kelowna BC V1V 1V7
    Canada
     

Patent:

  1. Patent for a process for the preparation of Cholera Vaccine (European Patent Office; Patent No.- 97309957.5-2105 on 19/05/98)
  2. Patent for a process for the isolation of a non-toxigenic Vibrio Cholerae strain. (European Patent Office; Patent No. - 97309957.5-2105 on 19/05/98
  3. International patent application: PCT patent application titled 'A Multi-Serotype Outer Membrane Vesicles (MOMV) of shigellae as a novel candidate vaccine' (PCT/IN2014/000369).
  4. Patent application for: "Alginate chitosan Nano formulation of Omp-A, a Shigella protein subunit vaccine" ICMR New Delhi. (Patent application # 201611015219/ IPR/NICED/OmpA/329 June, 2015)
     

Awards

  1. Young Scientist Award in IUPS International Congress 2001, Christchurch, New Zealand, August 26-31, 2001
  2. Invited Lecture, UGC Sponsored National Seminar, Dept. of Physiology, Presidency College, Kolkata, 19th December, 2008.
  3. JICA Fellowship : Worked with Dr. Shinji Yamasaki , Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1 Gakuen-cho, Nakaku, Sakai, Osaka Prefecture University, Osaka, Japan form 2007-09-19 to 2008-03-15
  4. Invited Oration Lecture: on "Dr. ( Mrs.) Chitralekha Mukherjee Memorial Oration-2010" at St. John's Medical College , Bangalore on 16th December 2010

Memberships

National Membership:

  1. Life member of Physiological Society of India
  2. Life member of Indian Science Congress Association

Foreign Membership:

  1. International Brain Research Organization (IBRO), Great Britain. Membership No. 1895-IBRO

Publications

2017

  1. Howlader DR, Koley H, Maiti S, Bhaumik U, Mukherjee P, Dutta S. A brief review on the immunological scenario and recent developmental status of vaccines against enteric fever. Vaccine. 2017 Nov 7;35(47):6359-6366.  Pubmed
  2. Mitobe J, Sinha R, Mitra S, Nag D, Saito N, Shimuta K, Koizumi N, Koley H. An attenuated Shigella mutant lacking the RNA-binding protein Hfq provides cross protection against Shigella strains of broad serotype. PLoSNegl Trop Dis. 2017 Jul 20;11(7 )   Pubmed
  3. Sinha R, Howlader DR, Ta A, Mitra S, Das S, Koley H. Retinoic acid pre-treatment down regulates V. cholerae outer membrane vesicles induced acute inflammation and enhances mucosal immunity. Vaccine. 2017 Jun16;35(28):3534-3547.    Pubmed

2016

  1. Ta A, Thakur BK, Dutta P, Sinha R, Koley H, Das S. Double-stranded RNA induces cathelicidin expression in the intestinal epithelial cells through phosphatidylinositol 3-kinase-protein kinase Cζ-Sp1 pathway and ameliorates shigellosis in mice. Cell Signal. 2017 Jul;35:140-153.  Pubmed
  2. Mandal RS, Ta A, Sinha R, Theeya N, Ghosh A, Tasneem M, Bhunia A, Koley H, Das S. Ribavirin suppresses bacterial virulence by targeting LysR-type transcriptional regulators. Sci Rep. 2016 Dec 19;6:39454.  Pubmed
  3. Roy S, Patra T, Golder T, Chatterjee S, Koley H, Nandy RK. Characterization of the gluconate utilization system of Vibrio cholerae with special reference to virulence modulation. Pathog Dis. 2016 Nov;74(8).  Pubmed
  4. Ghosh A, Koley H, Pal A. The Role of Vibrio choleraecholera Haemagglutinin Protease (HAP) in Extra-Intestinal Infection. J ClinDiagn Res. 2016 Sep;10(9)  Pubmed
  5. Chourashi R, Mondal M, Sinha R, Debnath A, Das S, Koley H, Chatterjee NS. Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis. Int J MedMicrobiol. 2016 Dec;306(8):657-665. Pubmed
  6. Bhattacharya D, Bhattacharya S, Patra MM, Chakravorty S, Sarkar S, ChakrabortyW, Koley H, Gachhui R. Antibacterial Activity of Polyphenolic Fraction of Kombucha Against Enteric Bacterial Pathogens. CurrMicrobiol. 2016  Pubmed
  7. Howlader DR, Sinha R, Nag D, Majumder N, Mukherjee P, Bhaumik U, MaitiS,Withey JH, Koley H. Zebrafish as a novel model for non-typhoidal Salmonella pathogenesis, transmission and vaccine efficacy. Vaccine. 2016 Sep30;34(42):5099-5106. Pubmed
  8. Sarkar P, Acharyya S, Banerjee A, Patra A, Thankamani K, Koley H, Bag PK. Intracellular, biofilm-inhibitory and membrane-damaging activities of nimbolide isolated from Azadirachta indica A. Juss (Meliaceae) against meticillin-resistant Staphylococcus aureus. J Med Microbiol. 2016 Oct;65(10):1205-1214. Pubmed
  9. Koley H, Ghosh P, Sinha R, Mitra S, Mukherjee P, Howlader DR, Chaki C, Nag D. Mice with Streptozotocin-Induced Hyperglycemia are Susceptible to Invasive Enteric Bacterial Infection. Jpn J Infect Dis. 2017 Jan 24;70(1):111-114. Pubmed

2015

  1. Nag D, Sinha R, Mitra S, Barman S, Takeda Y, Shinoda S, Chakrabarti MK, Koley H. 2015. Heat killed multi-serotype Shigellaimmunogens induced humoral immunity and protection against heterologous challenge in rabbit model. Immunobiology.220:1275-83. Pubmed
  2. Jaiswal A, Sarkar S, Das P, Nandy S, Koley H, Sarkar B. 2015. Trends in the genomic epidemiology of Vibrio cholerae O1 isolated worldwide since 1961. Int J Antimicrob Agents 46:460-4. Pubmed
  3. Withey JH, Nag D, Plecha SC, Sinha R, Koley H. 2015. Conjugated linoleic acid reduces cholera toxin production in vitro and in vivo by inhibiting Vibrio choleraeToxT activity. Antimicrob Agents Chemother. 59:7471-6. Pubmed
  4. Sinha R, Koley H, Nag D, Mitra S, Mukhopadhyay AK, Chattopadhyay B. 2015 Pentavalent outer membrane vesicles of Vibrio cholerae induce adaptive immune response and protective efficacy in both adult and passive suckling mice models. Microbes Infect. Mar;17(3):215-27. Pubmed
  5. Bhattacharya D, Dey S, Kadam S, Kalal S, Jali S, Koley H, Sinha R, Nag D, Kholkute SD, Roy S. 2015 Isolation of NDM-1-producing multidrug-resistant Pseudomonas putida from a paediatric case of acute gastroenteritis, India. New Microbes New Infect. Feb 28;5:5-9. Pubmed
  6. Rajpara N, Kutar BM, Sinha R, Nag D, Koley H, Ramamurthy T, Bhardwaj AK. 2015 Role of integrons, plasmids and SXT elements in multidrug resistance of Vibrio cholerae and Providenciavermicola obtained from a clinical isolate of diarrhea. Front Microbiol. Feb 17;6:57. Pubmed

2014

  1. Mondal M, D Nag, H Koley, DR Saha, Chatterjee NS. 2014. The Vibrio choleraeExtracellular Chitinase ChiA2 Is Important for Survival and Pathogenesis in the Host Intestine. PLoS One. 9:e103119. Pubmed
  2. Kanungo S, B Sen, T Ramamurthy, D Sur, B Manna, GP Pazhani, G Chowdhury, P Jhunjhunwala, RK Nandy, HKoley, MK Bhattacharya, S Gupta, G Goel, B Dey, M T, GB Nair, A Ghosh, D Mahalanabis.2014.Safety and immunogenicity of a live oral recombinant cholera vaccine VA1.4: a randomized, placebo controlled trial in healthy adults in a cholera endemic area in Kolkata, India. PLoS One. 9:e99381). Pubmed
  3. Barman S, H Koley, D Nag, S Shinoda, GB Nair, Y Takeda. 2014. Passive immunity with multi-serotype heat-killed Shigellae in neonatal mice. MicrobiolImmunol. 58:463-6. Pubmed
  4. Koley H, N Ray, G Chowdhury, S Barman, S Mitra, T Ramamurthy, AK Mukhopadhyay, BL Sarkar, R Katyal, P Das, S Panda, S Ghosh.2014. Outbreak of cholera caused by Vibrio cholerae O1 El Tor variant strain in Bihar, India. Jpn J Infect Dis.67:221-6. Pubmed
  5. Jaiswal A, H. Koley, S Mitra, DR Saha, B Sarkar. 2014.Comparative analysis of different oral approaches to treat Vibrio cholerae infection in adult mice. Int J Med Microbiol. 304:422-30. Pubmed
  6. Ghosh P, A Naha, S Basak, S Ghosh, T Ramamurthy, H. Koley, RK Nandy, S Shinoda, H Watanabe, AK Mukhopadhyay. 2014. Haitian variant tcpA in Vibrio cholerae O1 El Tor strains in Kolkata, India. J ClinMicrobiol.52:1020-1. Pubmed

2013

  1. Mitra S, MK Chakrabarti, H. Koley 2013 Multi-serotype outer membrane vesicles of Shigellae confer passive protection to the neonatal mice against shigellosis. Vaccine. 31: 3163-3173 Pubmed
  2. Sheikh IA, H. Koley, MK Chakrabarti, KM Hoque. 2013 The Epac1 signaling pathway regulates Cl- secretion via modulation of apical KCNN4c channels in diarrhea. J Biol Chem. 288: 20404-20415. Pubmed
  3. Das P, AK Singh, S Mukherjee, K Rajendran, DR Saha, H. Koley, S Basu. 2013 Composition of Escherichia coli population in the neonatal gut: phylogroups and virulence determinants. J Med Microbiol. 62: 1680-1687. Pubmed
  4. Mukherjee S, H. Koley, S Barman, S Mitra, S Datta, S Ghosh, D Paul, P Dhar. 2013 Oxalis corniculata (Oxalidaceae) leaf extract exerts in vitro antimicrobial and in vivo anticolonizing activities against Shigella dysenteriae 1 (NT4907) and Shigella flexneri 2a (2457T) in induced diarrhea in suckling mice. J Med Food. 16: 801-809 Pubmed
  5. Barman S, H. Koley, T Ramamurthy, MK Chakrabarti, S Shinoda, GB Nair, Y Takeda. 2013 Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model. Microbiol Immunol. 57: 762-771. Pubmed
  6. Rajpara N, K Vinothkumar, P Mohanty, AK Singh, R Singh, R Sinha, D Nag, H. Koley, A Kushwaha Bhardwaj. 2013 Synergistic Effect of Various Virulence Factors Leading to High Toxicity of Environmental V. cholerae Non-O1/ Non-O139 Isolates Lacking ctx Gene : Comparative Study with Clinical Strains. PLoS One. 8: e76200. Pubmed
  7. DR Saha, S Guin, R Krishnan, D Nag, H. Koley, S Shinoda, T Ramamurthy. 2013 Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies. Gut Pathog. 5: 36. Pubmed
  8. Jaiswal A, H. Koley, A Ghosh, A Palit, B Sarkar 2013 Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model. Microbes Infect. 15: 152-156. Pubmed

2012

  1. Mitra S, DR Saha, A Pal, Niyogi SK, U Mitra, H. Koley. 2012 Hemagglutinating activity is directly correlated with colonization ability of shigellae in suckling mouse model. Can J Microbiol. 58: 1159-1166. Pubmed
  2. Mitra S, S Barman, D Nag, R Sinha, DR Saha, H. Koley. 2012 Outer membrane vesicles of Shigella boydii type 4 induce passive immunity in neonatal mice. FEMS Immunol Med Microbiol. 66: 240-250. Pubmed
  3. China R, S Mukherjee, S Sen, S Bose, S Datta, H. Koley, S Ghosh, P Dhar. 2012 Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiol Res. 167: 500-506. Pubmed
  4. Patra T, H. Koley, T Ramamurthy, AC Ghose, RK Nandy. 2012 The Entner-Doudoroff pathway is obligatory for gluconate utilization and contributes to the pathogenicity of Vibrio cholerae. J Bacteriol. 194: 3377-3385. Pubmed
  5. Dey T K , S. Ghosh , M. Ghosh, H. Koley, P. Dhar 2012
    Comparative study of gastrointestinal absorption of EPA DHA rich fish oil from nano and conventional emulsion formulation in rats. Food Research International , 49: 72-79

2011

  1. Barman S, DR Saha, T Ramamurthy, H. Koley. 2011 Development of a new guinea-pig model of shigellosis. FEMS Immunol Med Microbiol. 62: 304-314. Pubmed
  2. Barman S, R Kumar, G Chowdhury, DR Saha, T Wajima, T Hamabata, R Tamamurthy, GB Nair, Y Takeda, H. Koley. 2011 A live noninvasive Shigella dysenteriae 1 strain induces homologous protective immunity in a guinea pig colitis model. Microbiol Immunol. 55, 683-693. Pubmed
  3. Mukherjee, Chaki S. Barman S., S Das., H. Koley and Dastidar Ghosh, 2011 Effective elimination of drug resistance genes in pathogenic Pseudomonas aeruginosa by antipsycholic agent thioridazine . Current Research in Bacteriology
  4. Ghosh S, Chakraborty K, Nagaraja T, Basak S, Koley H , Dutta S, Mitra U, Das S. 2011. An adhesion protein of Salmonella enterica serovar Typhi is required for pathogenesis and potential target for vaccine development. Proc Natl Acad Sci USA 108: 3348-3353. Pubmed
  5. Panda S, Pati KK, Bhattacharya MK, Koley H, Pahari S, Nair GB. 2011 Rapid situation response assessment of diarrhea outbreak in a coastal district following tropical cyclone AILA in India. Indian J Med Res. 133: 395-400. Pubmed

2010

  1. Roy, N., S Barman, A Ghosh, A Pal, K Chakraborty, SS Das, DR Saha, S Yamasaki, H. Koley. 2010. Immunogenicity and protective efficacy of Vibrio cholerae outer membrane vesicles in rabbit model. FEMS Immunol Med Microbiol. 60: 18-27. Pubmed
  2. Barman, S., S Chatterjee, G Chowdhury, T Ramamurthy, SK Niyogi, R Kumar, H. Koley 2010. Plasmid-mediated streptomycin and sulfamethoxazole resistance in Shigella flexneri 3a. Int J Antimicrob Agents. 36: 348-351. Pubmed
  3. Ghosh-Banerjee, J., M Senoh, T Takahashi, T Hamabata, S Barman, H. Koley, Mukhopadhyay AK, Ramamurthy T, Chatterjee S, Asakura M, Yamasaki S, Nair GB,Takeda Y. 2010 . Cholera toxin production by the El Tor variant of Vibrio cholerae O1compared to prototype El Tor and classical biotypes. J Clin Microbiol.48: 4283-6. Pubmed
  4. Syngkon A, S Elluri, H. Koley, P K Rompikuntal, DR Saha, MK Chakrabarti, RK Bhadra , SN Wai, A Pal. 2010 Studies on a novel serine protease of a ?hapA?prtV Vibrio cholerae O1 strain and its role in hemorrhagic response in the rabbit ileal loop model. PLoS One. 5(9). e13122. Pubmed

2009

  1. Shammas, M. A., Shmookler Reis RJ, H. Koley, Batchu RB, Li C, Munshi NC. 2009. Dysfunctional homologous recombination mediates genomic instability and progression in myeloma. Blood. 113: 2290-7. Pubmed
  2. Bhowmick, T.S., H. Koley, M. Das, D. R. Saha, B. L. Sarkar. 2009. Pathogenic potential of vibriophages against an experimental infection with Vibrio cholerae O1 in the RITARD model. Int J Antimicrob Agents. 33: 569-573. Pubmed
  3. Koley, H., S. Barman, N. Roy, D. R. Saha, R. Kumar. 2009. The efficacy and immunogenicity of a live transconjugant hybrid strain of Shigella dysenteriae type 1 in two animal models. World J. Microbiol Biotechnol. 25: 679-686. Pubmed

2008

  1. Chakraborty, K., Ghosh S, H. Koley, Mukhopadhyay AK, Ramamurthy T, Saha DR, Mukhopadhyay D, Roychowdhury S, Hamabata T, Takeda Y, Das S. 2008. Bacterial exotoxins downregulate cathelicidin (hCAP-18/LL-37) and human beta-defensin 1 (HBD-1) expression in the intestinal epithelial cells. Cell Microbiol. ;10: 2520-37. Pubmed
  2. Banerjee, S., Chattopadhyay R, Ghosh A, H. Koley, Panda K, Roy S, Chattopadhyay D, Chatterjee IB. 2008 .Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin C. J Inflamm 11; 5: 21. Pubmed
  3. Shammas, M. A., H. Koley, R. C. Bertheau, P. Neri, M. Fulciniti, P. Tassone, S. Blotta, A. Protopopov, C. Mitsiades, R. B. Batchu, K. C. Anderson, A. Chin, S. Gryaznov, N. C. Munshi. 2008. Telomerase inhibitor GRN163L inhibits myeloma cell growth in vitro and in vivo. Leukemia. 22:1410-8. Pubmed
  4. Koley, H., Soumik Barman, N. Roy, D. R. Saha, R. Kumar. 2008. The efficacy and immunogenicity of a live transconjugant hybrid strain of Shigella dysenteriae type 1 in two animal models. World J. Microbiol Biotechnol. 25: 679-686.
  5. Bhowmick, R., A. Ghosal, B. Das, H. Koley, D. R. Saha, S. Ganguly, R. K. Nandy, R. K. Bhadra, N. S. Chatterjee. 2008 Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin. Infect Immun. 76: 4968-77. Pubmed

2006

  1. Shammas, M. A., P. Neri, H. Koley, R. B. Batchu, R. C. Bertheau, V. Munshi, R. Prabhala, M. Fulciniti, Y. T. Tai, S. P. Treon, R. K. Goyal, K. C. Anderson, N. C. Munshi. 2006. Specific killing of multiple myeloma cells by (-) epigallocatechin-3-gallate extracted from green tea: biologic activity and therapeutic implications. Blood. 108: 2804-10. Pubmed
  2. Ghosh, A., D. R. Saha, K. M. Hoque, M. Asakuna, S. Yamasaki, H. Koley, S. S. Das, M. K. Chakrabarti, A. Pal. 2006. Enterotoxigenicity of mature 45-kilodalton and processed 35-kilodalton forms of hemagglutinin protease purified from a cholera toxin gene-negative Vibrio cholerae non-O1, non-O139 strain. Infect Immun. 74: 2937-46. Pubmed

2005

  1. Shammas, M. A., H. Koley, R. B. Batchu, R. C. Bertheau, A. Protopopov, N. C. Munshi, R. K. Goyal. 2005. Telomerase inhibition by siRNA causes senescence and apoptosis in Barrett's adenocarcinoma cells: mechanism and therapeutic potential. Mol. Cancer. 15: 4-24. Pubmed

2004

  1. Koley, H., M. A. Shammas, D. G. Beer, C. Li, R. K. Goyal, N. C. Munshi. 2004. Growth arrest, apoptosis and telomere shortening of Barrett's-associated adenocarcinoma cells by a telomerase inhibitor. Gastroenterology. 126: 1337-46. Pubmed
  2. Shammas, M. A., Reis R. J. Shmookler, C. Li, H. Koley, L. H. Hurley, K. C. Anderson, N. C. Munshi. 2004 Telomerase inhibition and cell growth arrest after telomestatin treatment in multiple myeloma. Clin Cancer Res. 10: 770-6. Pubmed

2003

  1. Shammas, M. A., Reis R. J. Shmookler, M. Akiyama, H. Koley, D. Chauhan, T. Hideshima, R. K. Goyal, L. H. Hurley, K. C. Anderson, N. C. Munshi. 2003. Telomerase inhibition and cell growth arrest by G-quadruplex interactive agent in multiple myeloma. Mol Cancer Ther. 2: 825-33. Pubmed

2002

  1. Shammas, M. A., H. Koley, R. K. Goyal and C. Nikhil. Munshi. 2002. Telomerase Inhibition and apoptotic cell growth arrest following telomestain treatment of multiple myeloma. Blood: 100, 11 810-15.

1999

  1. Thungapatra, M., C. Sharma, N. Gupta, R. K. Ghosh, A. Mukherjee, H. Koley, G. B. Nair and A. Ghosh. 1999. Construction of a recombinant live oral vaccine from a non-toxigenic strain in of V.cholerae O1 serotype Inaba biotype El Tor and assessment of its reactogenicity and immunogenicity in the rabbit model. Immunology Letter. 68: 219 -227. Pubmed
  2. Koley, H., Rupak Mitra, Arnab Basu, Asish K. Mukhopadhyay, P. K. Saha, B. S. Ramakrisha, Selvi Krishnan, Yoshifumi Takeda and G. Balakrish Nair. 1999. Response of Wild-type Mutants of Vibrio cholerae 01 possessing different combinations of Virulence zenes in the Ligated Rabbit Ileal Loop and in Ussing chambers: Evidence for the presence of additional secretogen. J. Med. Microbiol. 48: 51-57. Pubmed

1997

  1. Bhattacharya, S. K., M. K. Bhattacharya, D. Dutta, S. Dutta, M. Deb, A. Deb, A. G. Goswami, K .P. Das, H. Koley, G. B. Nair and A. Bagati. 1997 Double-blind, randomized clinical trial for safety and efficacy of Norfloxacin for shigellosis in children. Acta Paediatrica. 86: 319-320. Pubmed
  2. Saha, D. R., H. Koley, A. N. Ghosh and G. B. Nair. 1997. Sequential changes in gut mucosa of rabbits infected with Vibrio cholerae 0139 Bengal: an Ultrastructureal Study. J. Diarrhoeal. Dis. Res. 15: 59-64. Pubmed

1996

  1. Saha, P., H. Koley and G. B. Nair. 1996 Purification and Characterization of an extra cellular secretogenic non-membrane damaging cytotoxin produce by clinical strain of V.cholerae Non-01. Infection Immunity. 64: 3101-3108. Pubmed
  2. Saha, P., H. Koley, A. Mukherjee, S. K. Bhattacharya, G. B. Nair, B. S. Ramakrishna, S. Krishnan, Y. Takeda, 1996. Nontoxigenic Vibrio cholerae 01 Serotype Inaba Biotype EIT or Associated with a Cluster of Cased of Cholera in Southern India. Journal of Clinical Microbiology. 34: 1114-1117. Pubmed
  3. Sarkar, B. L., A. K. Chakrabarty, H. Koley, M. K. Chakrabarty and S. P. Dey. 1996. Biological activity and interaction of Vibrio cholerae bacteriopheges in rabbit ileal loop. Indian Journal of Medical Research. 104: 139-141. Pubmed
  4. Ghosh, A. R., M. Paul, H. Koley, S. K. Bhattacharya and G. B. Nair. 1996. Emergence of Shigella species in India. Lab. Medica. International. 5: 18-24.
  5. Ghosh, A. R., H. Koley, D. De, M. Paul, G. B. Nair and D. Sen. 1996. ETEC associated diarrhea among infants aged less than six months in Calcutta, India. European Journal of Epidemiology. 12: 81-84. Pubmed

1995

  1. Koley, H., A. N. Ghosh, M. Paul, A. R. Ghosh, P. K. Ganguly and G. B. Nair. 1995. Colonization ability and intestinal pathology of rabbits orally fed with Vibrio cholerae 0139 Bengal. Ind. J. Med. Res. 101: 57-60. Pubmed

1994

  1. Bhattacharya, M. K., S. K. Bhattacharya, M. Paul, D. Dutta, P. Dutta, H. Koley, D. De, A. R. Ghosh, P. Das and G. B. Nair. 1994. Shigellosis in Calcutta during 1990-1992. Antibiotic susceptibility pattern and clinical feature. J. Diarrhoeal Dis. Res. 12: 121-124. Pubmed
  2. Ghosh, A. R., H. Koley, D. De, S. Garg, M. K. Bhattacharya, S. K. Bhattacharya, G. B. Nair, T. Shimada, T. Takeda and Y. Takeda. 1994. Incidence and toxigenicity of Vibrio cholerae in a freshwater lake during the epidemic of cholera caused by serogroup O139 Bengal in Calcutta, India. FEMS Microbiol. Ecol. 14: 285-292.

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